Purification of CD451 hematopoietic cells directly from human bone marrow
Tuesday, 06 May 2008

Recently, a flow-based selectin-dependent method for capture and enrichment of specific type blood cells (CD341 hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD451 blood cells directly from human bone marrow to a high purity (90%–97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD451 cell enrichment procedures required for chimerism analysis following allogenic stem-cell transplantation. Am. J. Hematol. 00:000–000, 2008. Click here to read the full article.

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